Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (34): 5490-5495.doi: 10.3969/j.issn.2095-4344.0685

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Effects of two toothpastes used at different time on enamel remineralization and acid resistance

Zhao Yulong1, Yang Desheng2, Xu Fan1, Li Yanfeng3   

  1. 1Stomatological Hospital of Southern Medical University, Guangzhou 510000, Guangdong Province, China; 2Armed Police Force General Hospital, Beijing 100000, China; 3 First Affiliated Hospital of the PLA General Hospital, Beijing 100000, China
  • Received:2018-06-23 Online:2018-12-08 Published:2018-12-08
  • About author:Zhao Yulong, Master, Physician, Stomatological Hospital of Southern Medical University, Guangzhou 510000, Guangdong Province, China

Abstract:

BACKGROUND: Tooth brushing to reduce tooth wear is mostly recommended at 30 minutes after eating acidic food. However, little is reported on the effect of toothpastes used at different time on enamel remineralization.

OBJECTIVE: To evaluate the effect of bioactive glass toothpaste or fluoride toothpaste on enamel remineralization and acid resistance after remineralization when used instantly after being eroded or 30 minutes after being eroded.
METHODS: Fifty bovine enamel samples were randomly divided into five groups after the acid etching of carbonated beverage. The blank control group was placed in the natural saliva for 30 minutes. Instant fluoride group and instant bioactive glass group were immediately smeared with fluoride toothpaste and bioactive glass toothpaste, respectively, and then the samples in these two groups were placed in the natural saliva for 30 minutes. Groups of fluoride toothpaste or bioactive glass used at 30 minutes after acid etching were placed in the natural saliva for 30 minutes, and then the fluoride toothpaste or the bioactive glass toothpaste paste was applied respectively. After remineralization, all the samples were re-etched by carbonated beverages. After remineralization and re-etching, the surface microhardness of each sample was detected and observed by scanning electron microscope.

RESULTS AND CONCLUSION: (1) After remineralization, the surface microhardness of each group was higher than that in the blank control group (P < 0.05). The surface microhardness of the bioactive glass groups was higher than that in the fluoride groups (P < 0.05). The surface microhardness of the group with fluoride toothpaste used at 30 minutes after acid etching was higher than that in the instant fluorine group (P < 0.05). (2) After re-etching, the decrease in the surface microhardness was lower in the treatment groups than the blank control group (P < 0.05), and the decrease in the surface microhardness in the bioactive glass groups was lower than that in the fluoride groups (P < 0.05). (3) After remineralization and re-etching, most serious damage to the interprismatic substance was found in the blank control group, and the surface of the interprismatic substance was obviously lower than that of the enamel prisms. The flattest surface of the interprismatic substance in the bioactive glass groups was observed, and there was a minimal damage to the interprismatic substance and the enamel prisms. Moreover, a large number of spherical particles were attached to the surface of the interprismatic substance in the bioactive glass groups. To conclude, these results show that the bioactive glass can effectively enhance the remineralization and anti-corrosion ability of the etched enamel. The use of fluoride toothpaste instantly after acid etching can reduce the enamel remineralization. Moreover, there is no difference in enamel remineralization and acid resistance using bioactive glass instantly or 30 minutes after immersion in the natural saliva.

  中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Toothpastes, Tooth Remineralization, Dental Enamel, Acid Etching, Dental, Tissue Engineering

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